Tris Tricine SDS PAGE Stacking pH?
novalidaddress at nurfuerspam.de
Thu Aug 31 05:16:44 EST 2006
Dear Experts, I was following the recent discussion on native PAGE
stacking gel pH.
I frequently use Tris Tricine Gels and all protocols I am aware of use
the same buffer for stacking and separating gels, just different
concentrations of acrylamide. Is there a different physical chemistry
(ion mobilities) behind or do I have a bad protocol?
All input is welcome! Many thanks,
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