Tris Tricine SDS PAGE Stacking pH?

Wolfgang Schechinger novalidaddress at
Thu Aug 31 05:16:44 EST 2006

Dear Experts, I was following the recent discussion on native PAGE
stacking gel pH.

I frequently use Tris Tricine Gels and all protocols I am aware of use
the same buffer for stacking and separating gels, just different
concentrations of acrylamide. Is there a different physical chemistry
(ion mobilities) behind or do I have a bad protocol?

All input is welcome! Many thanks, 


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