(no subject)

[Mariana Coutinho]

Hi 
   
  You can raise polyclonal ascitys in mice which is easier to work with and results in and better antibody yeld. 
   
  Article: development of transplatable ascitys tumours wich continuously produce polyclonal antibodies in pristane primed BALB/c mice immunized with bacterial antigens and complete Freund adjuvant.
  Cevenini, R.  Journal of Immunological Methods 140 (1991) 111-118

  regards
   
  mariana
  
Dr Engelbert Buxbaum <engelbert_buxbaum At hotmail.com> escreveu:
  madhurima bhatnagar wrote:

> Hello to all
> I want to raise antibodies in mice for western blotting to detect
> expression level in my transgenic plants.Can anybody suggest me any
> references or protocols that can guide me in planning the experiments.

Mice are too small to raise an antiserum, they are used for monoclonals.
For serum, rabbits are better. Essential reading about immunological
methods is

@book{Har-88,
AUTHOR= {E. Harlow and D. Lane},
TITLE= {Antibodies --- A Laboratory Manual},
YEAR= {1988},
PUBLISHER= {Cold Spring Harbor Laboratory Press},
ADDRESS= {Cold Spring Harbor},
LANGUAGE= {engl}
}

_______________________________________________
Methods mailing list
Methods At net.bio.net
http://www.bio.net/biomail/listinfo/methods


 		
---------------------------------
 Yahoo! Search
 Música para ver e ouvir: You're Beautiful, do James Blunt