sequencing prediction

Jinchun Wang via methods%40net.bio.net (by jwang24 At mail.uh.edu)
Sat Dec 2 23:29:17 EST 2006


hi everyone,

I have 20 different DNA duplexs in a mixture. After ligation, transformation, I got a plate of colonies. Now how can I predict how many colonies should be sequenced to find all 20 DNA?  If I already sequenced 100 colonies and got 12 DNA, is there some model to calculate how many more should be sequenced to find others?

Many thanks 
 				
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Jinchun Wang
2006-12-02



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