sequencing prediction

Jinchun Wang via (by jwang24 At
Sat Dec 2 23:29:17 EST 2006

hi everyone,

I have 20 different DNA duplexs in a mixture. After ligation, transformation, I got a plate of colonies. Now how can I predict how many colonies should be sequenced to find all 20 DNA?  If I already sequenced 100 colonies and got 12 DNA, is there some model to calculate how many more should be sequenced to find others?

Many thanks 
Jinchun Wang

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