SDS PAGE

[Tom Anderson]

On Tue, 5 Dec 2006, Dr Engelbert Buxbaum wrote:

> younes sadramehr wrote:
>
> >   We had run a total protein SDS PAGE . After staining we saw a new &
> > different band in one of the PAGE columns . Now we want to
> > repurificate the protein ( the new band ) from the poly acrylamide gel
>
> If you don't have elecroelution facilities you can press the gel slice
> through a 27G needle and incubate the slurry with buffer solution in an
> end-over-end mixer overnight. 10 mM Bicarbonate pH 8 or similar should
> do. Spin the gel fragments down and withdraw the supernatant.

A colleague of a colleague used to do this by cutting the end off a 1000
ul filter tip, putting it in a microtube, putting the gel piece on top of
the filter, and spinning it. Apparently this works, although i dread to
think what the yield is like.

tom

-- 
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264   (f) +44 (20) 76797805   (e) thomas.anderson At ucl.ac.uk