Plasmid as a template PCR

Pow Joshi via methods%40net.bio.net (by pow.joshi At gmail.com)
Wed Dec 6 11:15:52 EST 2006


On 6 Dec 2006 02:57:19 -0800, ati405 At gmail.com <ati405 At gmail.com> wrote:
> Hi
> I cloned one beta-thalassemia mutation (Homo) in t-vector
> but when I do ARMS-PCR ....I see 2 band...one for mutant and another
> for noraml !!!!!
> I have to see only one band for mutant gene!!
> I change several factors...but it reapeat....
> plaese help me...
> It's very important!!!


you may be having some contamination problem.... could you give more
details about the where you isolate the plasmid from, etc.,
pow
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