Plasmid as a template PCR

Pow Joshi via (by pow.joshi At
Wed Dec 6 11:15:52 EST 2006

On 6 Dec 2006 02:57:19 -0800, ati405 At <ati405 At> wrote:
> Hi
> I cloned one beta-thalassemia mutation (Homo) in t-vector
> but when I do ARMS-PCR ....I see 2 for mutant and another
> for noraml !!!!!
> I have to see only one band for mutant gene!!
> I change several factors...but it reapeat....
> plaese help me...
> It's very important!!!

you may be having some contamination problem.... could you give more
details about the where you isolate the plasmid from, etc.,
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