Fwd: R: EtBr decontamination

Pow Joshi via methods%40net.bio.net (by pow.joshi At gmail.com)
Thu Dec 7 16:44:54 EST 2006

---------- Forwarded message ----------
From: Pow Joshi <pow.joshi At gmail.com>
Date: Thu, 7 Dec 2006 12:13:09 -0500
Subject: Re: R: EtBr decontamination
To: "Prof. Piero Sestili" <sestili At uniurb.it>

On 12/7/06, Prof. Piero Sestili <sestili At uniurb.it> wrote:
> I feel safe with EtBr trapping bags from sigma. As an alternative I use
> to bleach EtBr in concentrated hypochlorite and as color disappears I
> also add HCl since I had seen somewhere that acidic environment promotes
> degradation of residual dangerous byproducts.  This procedure was on the
> WEB so you can look with a search engine for further details,
> Piero

Thank you Piero,  and Han, for the inputs...I did try the
google...(always do before I cry for help ;)); however, the data on
google confused me. I shall try the activated charcoal procedure.
 However, my major concern is a little wierd: We wash out own lab
coats, and I was wondering how safe that was to use with bleach in the
household-washing machine...

thank you again,
> Prof. Piero Sestili
> Istituto di Farmacologia e Farmacognosia e
> Centro di Ricerca sull'Attività Motoria
> Università degli Studi di Urbino "Carlo Bo"
> Via "I Maggetti" 26
> 61029 URBINO (PU)
> Tel. 0722 303414; 0722 305524
> Fax 0722 303401
> -----Messaggio originale-----
> Da: methods-bounces At oat.bio.indiana.edu
> [mailto:methods-bounces At oat.bio.indiana.edu] Per conto di Pow Joshi
> Inviato: giovedì 7 dicembre 2006 0.27
> A: Methods At magpie.bio.indiana.edu
> Oggetto: EtBr decontamination
> Hi everyone,
> I was wondering if anyone would know the details of decontaminating
> EtBr;  and if it interacts with Bleach to form any kind of toxic
> intermediate or product.
> thanks
> pow
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