How to inactivate proteins in organ extracts?

Michael Sullivan via methods%40net.bio.net (by mlsulliv At wisc.edu)
Fri Dec 8 10:00:22 EST 2006


Instead of trypsin, you might use proteinase K instead. I think this  
is very typically used to deproteinize extracts.

Mike Sullivan

On Dec 7, 2006, at 4:03 PM, WS wrote:

> Dear Experts!
>
> How to inactivate proteins in an aqueous organ extract? An attempt to
> digest them with trypsin failed, probably due to trypsin inhibitor  
> from
> blood / serum present in the samples. Heating / boiling comes into my
> mind, but this might kill other unknown substances in the extract.
>
> I would like to tell if a certain effect of this extract is due to a
> protein or something else.
>
> Any ideas?
>
> Thanks for your input!
>
> Wolfgang
>
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---
Michael L. Sullivan
Plant Research Molecular Geneticist
US Dairy Forage Research Center
ARS-USDA
1925 Linden Drive West
Madison, WI 53706
(608) 890-0046 (Phone)
(608) 890-0076 (FAX)



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