How to inactivate proteins in organ extracts?
(by mlsulliv At wisc.edu)
Fri Dec 8 10:00:22 EST 2006
Instead of trypsin, you might use proteinase K instead. I think this
is very typically used to deproteinize extracts.
On Dec 7, 2006, at 4:03 PM, WS wrote:
> Dear Experts!
> How to inactivate proteins in an aqueous organ extract? An attempt to
> digest them with trypsin failed, probably due to trypsin inhibitor
> blood / serum present in the samples. Heating / boiling comes into my
> mind, but this might kill other unknown substances in the extract.
> I would like to tell if a certain effect of this extract is due to a
> protein or something else.
> Any ideas?
> Thanks for your input!
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Michael L. Sullivan
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US Dairy Forage Research Center
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