Ligation problems

Vural, Ali via methods%40net.bio.net (by AVural At lsuhsc.edu)
Fri Dec 8 14:22:09 EST 2006


Hi again,

 

My another question will be about adding nucleotides to the ligation
reaction. So what should be the amount of nucleotides for a 10 ul
ligation rxn. I am looking forward to hear from you. i think this is
going to be my last try for my ligation experiment. It has been very
long time I am trying to clone my fragment into vector. Thank you for
your help. 

 

Ali



More information about the Methods mailing list