Ligation problems

Vural, Ali via (by AVural At
Fri Dec 8 14:22:09 EST 2006

Hi again,


My another question will be about adding nucleotides to the ligation
reaction. So what should be the amount of nucleotides for a 10 ul
ligation rxn. I am looking forward to hear from you. i think this is
going to be my last try for my ligation experiment. It has been very
long time I am trying to clone my fragment into vector. Thank you for
your help. 



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