How to inactivate proteins in organ extracts?

Nick Theodorakis via methods%40net.bio.net (by nick_theodorakis At hotmail.com)
Sat Dec 9 21:19:01 EST 2006


WS wrote:
> Dear Michael,
>
> How may I inactivate proteinase K (except heating to the boil)? I would
> like to apply the digested extract to my cells.
>


Serine protease inhibitors such as PMSF or DIFP inhibit Proteinase K.

Nick

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