Proteins

Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum At hotmail.com)
Sun Dec 10 03:36:21 EST 2006


camelia vlad wrote:
  
>   Please send me the protocol for proteins intact extraction from
> gel

For your application this may not be required. You can incubate one lane
of the gel directly with SDS-sample buffer and then place it onto the
second dimension gel (i.e. do a 2D-electrophoresis just with
non-reducing PAGE rather than IEF as first dimension).

If you stain with one of those fluorescent dyes like nile red or SYPRO
Red that do not require fixing you could also cut out just the band you
are interested in, incubate that with sample buffer and place it into
the well of another gel.


More information about the Methods mailing list