Ligation problems

Vural, Ali via methods%40net.bio.net (by AVural At lsuhsc.edu)
Mon Dec 11 16:34:19 EST 2006


The ends are cohesive and at the end I did not get various lengths. All
the bands corresponding to vector insert after restriction enzyme
screening was uniform and shorter that its actual length whereas the
insert was okay. And I am sure from the length of the vector insert
since it ligated successfully to another insert without any truncation. 



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