(by AVural At lsuhsc.edu)
Mon Dec 11 16:34:19 EST 2006
The ends are cohesive and at the end I did not get various lengths. All
the bands corresponding to vector insert after restriction enzyme
screening was uniform and shorter that its actual length whereas the
insert was okay. And I am sure from the length of the vector insert
since it ligated successfully to another insert without any truncation.
More information about the Methods