non specific amplification in clone fragment using primer aganist
vector
muhammad yasir
via methods%40net.bio.net
(by yasirphr At yahoo.com)
Sat Dec 16 02:05:39 EST 2006
i have constructed 16s rRNA library in topo TA cloning vectore of invetroge and desigen primer for vector region near to the cloning site. but in the screening i am getting one light band that is extactly double (3kb) of the clone fragment (1.5Kb). i shall be very glad to know from some one, why i am getting this non specific amplification and how to solve this problem.
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