Hi i need Plasmid containing taq gene
WS
via methods%40net.bio.net
(by novalidaddress At nurfuerspam.de)
Sat Dec 16 18:16:56 EST 2006
I there is npbody who has it or wants to share it:
1) maybe you are lucky to obtain some Thermus aquaticus or its RNA or
DNA
2)
http://www.genome.jp/dbget-bin/www_bfind_sub?dbkey=genbank-today&keywords=thermus+aquaticus+dna+polymerase&mode=bfind&max_hit=1000
reveals the sequence of Taq
3) make primers, , maybe add some tags for purification, clone it into
your favorite expression vector
4) purify a little (or slave some students)
Have fun!
Wo
Jose de las Heras wrote:
> it's not unusual for researches to make their own Taq, nothing wrong with
> that.
>
> Jose
>
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