Hi i need Plasmid containing taq gene

[Christian Praetorius]

"Jose de las Heras" <josenet At tiscali.co.uk> wrote:

>In our lab we have prepared our own Taq, in a much cruder way, but it works. 
>Essentially it comes down to making a protein extract froma  bug that 
>contains an expression vector with the Taq in it... after induction you 
>produce loads of it, as seen on a gel simply by Coomasie staining. We 

I once worked in a lab, where they had a similiar approach.
Overexpression in E.coli, lysis of the cells, the supernatant was
boiled for approximately an hour at 72°C, centrifuged, the supernatant
seperated and then the Taq was precipitated with ammonium sulfate,
concentrated and resuspended. I don't have any exakt protocol, since I
was not involved in the procedure, but the Taq worked quite well.

Christian

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