lysis buffer for membrane bounded protein

Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum At hotmail.com)
Mon Dec 18 15:02:49 EST 2006


Paras Anand wrote:

> Can someone tell me about the best way to remove proteins that are 
> loosely associated to the plasma membrane.

Classical extraction media are 250 mM KI (Michelis & Spanswick, Plant
Physiol. 81 (1986) 542-7) or 100 mM Na2CO3 (Fujiki et al., J. Cell Biol.
93 (1982) 97-102).


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