Which HRP substrate.

Louis Hom lhom at OCF.Berkeley.EDU
Wed Feb 1 08:14:26 EST 2006


In article <mailman.863.1138295376.29584.methods at net.bio.net>,
Matthew Davies <m.davies at herts.ac.uk> wrote:
>
>I am not a biologist or even a biochemist.  I am a chemist trying to use =
>HRP
>as part of a system to quantitatively detect the formation of hydrogen
>peroxide.

	I don't have any firsthand experience with it, but the Worthington 
Enzymes manual (info may be freely available at their website) describes 
an assay using 4-aminoantipyrene (absorbance at 510, rxn @ pH 7.0).
-- 
______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/ 	    


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