Which HRP substrate.
Louis Hom
lhom at OCF.Berkeley.EDU
Wed Feb 1 08:14:26 EST 2006
In article <mailman.863.1138295376.29584.methods at net.bio.net>,
Matthew Davies <m.davies at herts.ac.uk> wrote:
>
>I am not a biologist or even a biochemist. I am a chemist trying to use =
>HRP
>as part of a system to quantitatively detect the formation of hydrogen
>peroxide.
I don't have any firsthand experience with it, but the Worthington
Enzymes manual (info may be freely available at their website) describes
an assay using 4-aminoantipyrene (absorbance at 510, rxn @ pH 7.0).
--
______________________________________________________________________________
Lou Hom >K'93
lhom at ocf.berkeley.edu
http://www.ocf.berkeley.edu/~lhom/
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