Ammonium or Potassium Acetate?

J Winkler news.100.johanneswinkler at spamgourmet.com
Fri Feb 3 20:25:27 EST 2006


J Winkler wrote:
> Wolfgang Schechinger wrote:
> 
>> Dear experts,
>>
>> some miniprep protocols use ammonium acetate instead of potassium
>> acetate. Is there any advantage or is this just another variation of
>> the theme?
> 
> 
> Ammonium acetate (typically at a final concentration of 2.5 M, e.g. 
> Morelle (1980) in Focus 11(1)) supposedly achieves better deproteination 
> of the lysate.
Sorry, the year for this one must read 1989, not 1980. Regarding 
deproteination, it refers to Crouse & Amourese (1987), Focus 9(2), p.3. 
Both Focus issues are available for download from Invitrogen, btw.


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