expression vector pSVT7

Nick Theodorakis nick_theodorakis at hotmail.com
Fri Feb 3 18:15:14 EST 2006


J Winkler wrote:
[...]

> Construction of pSVT7 is described in 
> http://www.jcb.org/cgi/reprint/105/6/2905 - sorry, no sequence
> info there. The problem with all these "old" vectors is that very 
> little, if any, sequence info is available (and probably these vectors 
> were never fully sequenced in the first place).
> 
> Johannes

Sometimes if you have enough detail on the way a plasmid was 
constructed, it's possible to reconstruct a map (or even a sequence, if 
you have a good plasmid management software). We used to call this 
"plasmid archeology."

Nick


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Nick Theodorakis
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