Ammonium or Potassium Acetate?

Duncan Clark blackhole at abuse.plus.com
Mon Feb 6 04:08:03 EST 2006


Historians believe that in newspost 
<mailman.998.1139078675.29584.methods at net.bio.net> on Sat, 4 Feb 2006, 
Dr. Hiranya S. Roychowdhury <hroychow at nmsu.edu> penned the following 
literary masterpiece:
>Ammonium is to be avoided if
>you are using the DNA for ligation, since Ammonium ions seem to interfere with
>the reaction.

I don't know about it's inhibition in ligation but ammonium ions should 
be avoided in EtOH pptations if subsequently using T4PNK.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.


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