Ammonium or Potassium Acetate?
Dr. Hiranya S. Roychowdhury
hroychow at nmsu.edu
Mon Feb 6 15:45:52 EST 2006
You are right ... it is the kinasing reaction, not the ligation reaction.
Quoting Duncan Clark <blackhole at abuse.plus.com>:
> Historians believe that in newspost
> <mailman.998.1139078675.29584.methods at net.bio.net> on Sat, 4 Feb 2006,
> Dr. Hiranya S. Roychowdhury <hroychow at nmsu.edu> penned the following
> literary masterpiece:
> >Ammonium is to be avoided if
> >you are using the DNA for ligation, since Ammonium ions seem to interfere
> >the reaction.
> I don't know about it's inhibition in ligation but ammonium ions should
> be avoided in EtOH pptations if subsequently using T4PNK.
> I love deadlines. I especially like the whooshing noise they make as
> they go flying by.
> Duncan Clark
> GeneSys Ltd.
> Methods mailing list
> Methods at net.bio.net
Hiranya S. Roychowdhury, Ph.D.
Dept. of Health and Public Services
Dona Ana Branch Community College
New Mexico State University
Las Cruces, NM 88003
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