Ammonium or Potassium Acetate?

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Mon Feb 6 15:45:52 EST 2006


You are right ... it is the kinasing reaction, not the ligation reaction.



Quoting Duncan Clark <blackhole at abuse.plus.com>:

> Historians believe that in newspost 
> <mailman.998.1139078675.29584.methods at net.bio.net> on Sat, 4 Feb 2006, 
> Dr. Hiranya S. Roychowdhury <hroychow at nmsu.edu> penned the following 
> literary masterpiece:
> >Ammonium is to be avoided if
> >you are using the DNA for ligation, since Ammonium ions seem to interfere
> with
> >the reaction.
> 
> I don't know about it's inhibition in ligation but ammonium ions should 
> be avoided in EtOH pptations if subsequently using T4PNK.
> 
> Duncan
> -- 
> I love deadlines. I especially like the whooshing noise they make as
> they go flying by.
> 
> Duncan Clark
> GeneSys Ltd.
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> Methods at net.bio.net
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> 


-- 
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Dept. of Health and Public Services
Dona Ana Branch Community College
Rm# 191T, 
New Mexico State University
Las Cruces, NM 88003



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