lambda exonuclease with aa-dUTP

Susan Hogarth hogarth at gmail.com
Tue Feb 7 18:55:31 EST 2006


I'm thinking about generating ssDNA from a PCR reaction using lambda
exonuclease. The PCR reaction incorporates aa-dUTP into the product
(for subsequent coupling with Cy dyes). Does anyone have any knowledge
or speculation about how lambda exonuclease would handle aa-dUTPs when
it hit them? Would it cause the reaction to terminate?

- Susan



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