Guaiacol as HRP substrate
m.davies at herts.ac.uk
Thu Feb 9 07:48:24 EST 2006
Recently enquired about substrates for HRP. Decided on Guaiacol
(2-methoxyphenol) after reviewing the suggestions (thanks all).
Unfortunately, when I try the reaction between H2O2 and Guaiacol with =
get a dark brown precipitate. I=92m using a phosphate buffer at pH 7. =
changed from potassium to sodium based phosphate buffer to avoid
precipitation, but its still occurring. The product is supposed to be
3,3=92-dimethoxy-4,4=92-biphenoquinone. I=92m not using excessive =
substrate (6mM) or H2O2 (bout 3 mM). Have reviewed my experimental
procedure and it doesn=92t appear to be too different from other =
Any help is much appreciated. Thanks all.
Science & Technology Research Institute
University of Hertfordshire
No virus found in this outgoing message.
Checked by AVG Free Edition.
Version: 7.1.375 / Virus Database: 267.15.3/254 - Release Date: =
More information about the Methods