flanking 5'-bases on BglI site within a primer
Fernando
king-ferdi at gmx.net
Thu Feb 9 12:13:37 EST 2006
according to fermentas there should be no problem if more than two
additional bases were added...
http://www.fermentas.com/techinfo/re/restrdigpcrii.htm
Josmar wrote:
> Dear all,
> does anyone have experience how many flanking 5'-bases are needed on
> primers that were designed to introduce BglI sites into a pcr product?
> We have good evidence that our pcr-product with 5 additional bases is
> only very poorly cut, even overnight. We kind of rely on a
> quantitatively cut product because it contains a library. The NEB
> catalogue does not give a hint on this. I guess we will try 10
> additional bases next.
> Cheers
> Josmar
>
More information about the Methods
mailing list