flanking 5'-bases on BglI site within a primer
news.100.johanneswinkler at spamgourmet.com
Thu Feb 9 14:57:01 EST 2006
> Page 258 in the NEB 2005-2006 catalog has info on cleavage close to the
> end of fragments. For Bgl II they got 100% cleavage with 3 bp extra.
> Josmar wrote:
>> does anyone have experience how many flanking 5'-bases are needed on
>> primers that were designed to introduce BglI sites into a pcr product?
The query was about BglI, not II. Sorry, I don't have personal
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