flanking 5'-bases on BglI site within a primer

J Winkler news.100.johanneswinkler at spamgourmet.com
Thu Feb 9 14:57:01 EST 2006


Allison wrote:
> Page 258 in the NEB 2005-2006 catalog has info on cleavage close to the 
> end of fragments.  For Bgl II they got 100% cleavage with 3 bp extra. 

> Josmar wrote:
...
>> does anyone have experience how many flanking 5'-bases are needed on
>> primers that were designed to introduce BglI sites into a pcr product?

The query was about BglI, not II. Sorry, I don't have personal 
experiences either.

Johannes


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