Methods Digest, Vol 9, Issue 12

Siddhartha Parida sidharth4 at gmail.com
Tue Feb 14 00:25:04 EST 2006


 Dear Dr.Quang


The CTAB method for isolation of plant DNA is an excellent method, you
can use proteinase K but without it also the amount of DNA extracted
is good

Do mention the forest species which u want to work with ? i can send u
the protocol



On 2/13/06, methods-request at oat.bio.indiana.edu
<methods-request at oat.bio.indiana.edu> wrote:
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> Today's Topics:
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>   1. Re: Pen to mark molecular weight standards on radioactive
>      blots ??? (Dr Engelbert Buxbaum)
>   2. DNA isolation by CTAB with Proteinase-K for plant tissue?
>      (TRAN HO QUANG)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 07 Feb 2006 16:32:03 +0100
> From: Dr Engelbert Buxbaum <engelbert_buxbaum at hotmail.com>
> Subject: Re: Pen to mark molecular weight standards on radioactive
>        blots ???
> To: methods at net.bio.net
> Message-ID: <dsaehk$r0k$00$1 at news.t-online.com>
> Content-Type: text/plain; charset=iso-8859-2
>
> josmarlangner at yahoo.de wrote:
>
> > Hi there,
> > could anyone please give a hint where to purchase pens to mark
> > unlabelled molecular weight standards on radioactive blots? I don't
> > think the ink is radioactive but it somehow gives a signal on the film
> > (chemolumi?). I remember that I ordered such a pen about ten years ago
> > but I don't remember where.
>
> I got mine from BellArt Products, California (usual disclaimer applies).
> The thing is non-radioactive, but contains a phosphorescent dye instead.
> After exposure to ambient light it will glow for a while, long enough to
> leave a "lasting impression" on X-ray film.
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 13 Feb 2006 00:30:32 -0800 (PST)
> From: TRAN HO QUANG <quangtranho at yahoo.com>
> Subject: DNA isolation by CTAB with Proteinase-K for plant tissue?
> To: methods at magpie.bio.indiana.edu
> Message-ID: <20060213083032.16414.qmail at web34206.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi netters,
>
> Does anyone have experiences using CTAB method with
> Proteinase-K for DNA isolation of plant tissue,
> particularly for forest tree species?
>
> Anny suggestions are appreciated.
>
>
>
> -------------------------------------------
> Tran Ho Quang
> Research Centre for Forest Tree Improvement
> (Forest Science Institute of Vietnam)
> Dong Ngac - Tu Liem - Hanoi. Vietnam
> Tel; 84-4-8389813     Fax; 84-4-8362280
>
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> End of Methods Digest, Vol 9, Issue 12
> **************************************
>


--
Sidharth Parida
Senior Faculty
Center for biotechnology and Research
Neelachal Inst of Medical Sciences
O.C.H.C building , Near Ram Mandir
Bhubaneswar-3
ORISSA
09437089337(M)
sidharth4 at gmail.com



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