UV light kills DNA contamination in PCR samples

Jayakumar, R R.Jayakumar at roswellpark.org
Sun Feb 19 16:58:00 EST 2006


If UV did not affect the single stranded PCR primers, does that also
mean that this technique can be used to eliminate and DNA contamination
in RNA preps (traditionally RNASE free DNASE is used for this purpose)?
    
Jai

-----Original Message-----
From: methods-bounces at oat.bio.indiana.edu
[mailto:methods-bounces at oat.bio.indiana.edu] On Behalf Of x j
Sent: Saturday, February 18, 2006 9:28 PM
To: methods at magpie.bio.indiana.edu
Subject: UV light kills DNA contamination in PCR samples

Dear Sir/Maddam,
   
  I found the following message from internet. Could you plase give me
the volumn, pages of the article that appeared on Nature. Thanks, Jun
  ---------------- 
  A friend of mine found this in some lab journal, thought I'd pass it
on to you...
***
Shining UV light on PCR reagents can eliminate DNA contamination,
according to a letter in the January 4 issue of Nature.  When
researchers
at the Mayo Clinic/Foundation (Rochester, MN) took PCR mix containing
between 3 and 30,000 pg of DNA, irradiated it with 254- and 300-nm bulbs
for 5 or 20 minutes, and amplified it for 30 cycles, no amplified
product
was observed.  Even 3 pg of starting DNA normally gives a detectable
amount of product after 30 cycles of amplification.  DNA added to an
irradiated PCR mix could still be amplified, indicating that UV
treatment
didn't affect the reagent's ability to amplify.  Although a 5-minute
irradiation reduced the amplification efficiency of double-stranded DNA,
single-stranded primers in the PCR mix still maintained their integrity.
***
dan


		
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