Methods Digest, Vol 9, Issue 18

Siddhartha Parida sidharth4 at gmail.com
Sun Feb 19 23:40:17 EST 2006


Etbr is its stock solution(10mg/ml) form is to some extent harmful but
not when used in gels or staining buffer , u can just wash ur hands if
u get in touch

On 2/19/06, methods-request at oat.bio.indiana.edu
<methods-request at oat.bio.indiana.edu> wrote:
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> Today's Topics:
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>   1. ethidium bromide contamination on clothes (el)
>   2. Re: ethidium bromide contamination on clothes (DK)
>   3. Re: ethidium bromide contamination on clothes (Aawara Chowdhury)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: 17 Feb 2006 20:35:57 -0800
> From: "el" <elsbeau3 at yahoo.com>
> Subject: ethidium bromide contamination on clothes
> To: methods at net.bio.net
> Message-ID: <1140237357.755607.66830 at f14g2000cwb.googlegroups.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> the other day i was transfering a gel from the eletrophoresis box to a
> tray to take it to the UV light box (while wearing gloves ofcourse)and
> accidentally spilled some buffer on the front of my lab coat. I changed
> my lab coat and went about the lab doing some stuff. then i realized
> that some buffer may have gotten on my pants but wasn't really
> concerned about it because i didn't realize that the buffer became
> contaminated with ethidium bromide while you run it (i just assumed it
> stayed in the gel). The amount in the gel was probably 1 to 0.5
> micrograms per ml when i found out a couple of days later that there
> was a small amount of ethidium bromide in the buffer i examined the lab
> coat on the UV light box and there didn't appear to be any
> contamination (ie there was no visible florescence where the buffer had
> been spilled). I consulted the MSDS about my jeans and  it just said to
> wash contaminated clothing before reuse so i threw my jeans in the wash
> with regular detergent separately for a 30 minute cycle. I assumed this
> would take care of removing any residual ethidium bromide but now i am
> worried that i didn't based upon looking online.  the jeans (after
> being washed) got mixed up with my other laundry and went into the
> dryer with them when i meant to keep them separate. after being
> paranoid i just threw the jeans out but could i have contaminated the
> rest of my clothes with ethidium bromide or should i not worry about it
> because it was such a small amount???
>
>
>
> ------------------------------
>
> Message: 2
> Date: Sat, 18 Feb 2006 04:48:24 GMT
> From: dk at no.email.thankstospam.net (DK)
> Subject: Re: ethidium bromide contamination on clothes
> To: methods at net.bio.net
> Message-ID: <dt68un$6qn$1 at news.doit.wisc.edu>
>
> In article <1140237357.755607.66830 at f14g2000cwb.googlegroups.com>, "el" <elsbeau3 at yahoo.com> wrote:
> >the other day i was transfering a gel from the eletrophoresis box to a
> >tray to take it to the UV light box (while wearing gloves ofcourse)and
> >accidentally spilled some buffer on the front of my lab coat. I changed
> >my lab coat and went about the lab doing some stuff. then i realized
> >that some buffer may have gotten on my pants but wasn't really
> >concerned about it because i didn't realize that the buffer became
> >contaminated with ethidium bromide while you run it (i just assumed it
> >stayed in the gel). The amount in the gel was probably 1 to 0.5
> >micrograms per ml when i found out a couple of days later that there
> >was a small amount of ethidium bromide in the buffer i examined the lab
> >coat on the UV light box and there didn't appear to be any
> >contamination (ie there was no visible florescence where the buffer had
> >been spilled). I consulted the MSDS about my jeans and  it just said to
> >wash contaminated clothing before reuse so i threw my jeans in the wash
> >with regular detergent separately for a 30 minute cycle. I assumed this
> >would take care of removing any residual ethidium bromide but now i am
> >worried that i didn't based upon looking online.  the jeans (after
> >being washed) got mixed up with my other laundry and went into the
> >dryer with them when i meant to keep them separate. after being
> >paranoid i just threw the jeans out but could i have contaminated the
> >rest of my clothes with ethidium bromide or should i not worry about it
> >because it was such a small amount???
>
> No need to be that paranoid. EthBr is very soluble and was washed
> away in your washer just fine. Besides, the amounts you are talking
> about are incredibly negligible to begin with.
>
> And that is not to mention that EthBr is not particularly mutagenic and
> it's carcinogenicity is entirely putative. A single smoked sigarette
> contains 100X more mutagenes than the amount of EthBr you are
> talking about.
>
> DK
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Sat, 18 Feb 2006 05:19:37 GMT
> From: Aawara Chowdhury <aawara at FEMA-trailer.org>
> Subject: Re: ethidium bromide contamination on clothes
> To: methods at net.bio.net
> Message-ID: <J7yJf.48257$697.15457 at bignews3.bellsouth.net>
>
> In <dt68un$6qn$1 at news.doit.wisc.edu>,
>  DK <dk at no.email.thankstospam.net> wrote:
>
> > No need to be that paranoid. EthBr is very soluble and was washed
> > away in your washer just fine. Besides, the amounts you are talking
> > about are incredibly negligible to begin with.
> >
> > And that is not to mention that EthBr is not particularly mutagenic and
> > it's carcinogenicity is entirely putative. A single smoked sigarette
> > contains 100X more mutagenes than the amount of EthBr you are
> > talking about.
>
> Does ethidium bromide even cross the plasma membrane of intact cells?
>
> AC
> --
> Email: echo 142322093203359315271794620168064975321554275890186P | dc
>
>
> ------------------------------
>
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> End of Methods Digest, Vol 9, Issue 18
> **************************************
>


--
Sidharth Parida
Senior Faculty
Center for biotechnology and Research
Neelachal Inst of Medical Sciences
O.C.H.C building , Near Ram Mandir
Bhubaneswar-3
ORISSA
09437089337(M)
sidharth4 at gmail.com



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