pH adjustment in Tris-HCl buffer?

Louis Hom lhom at OCF.Berkeley.EDU
Fri Feb 24 08:50:25 EST 2006

I'd say the preferred method is to start with Tris base and then adjust 
downward with HCl.  Alternatively, you could take a solution of Tris-HCl 
and a solution of Tris base of the same concentration and mix them until 
you reach the desired pH.  Adding NaOH (essentially backtitrating an 
overshot) leads to a higher concentration of NaCl than you intend, which 
may or may not have actual effects on your experiment.
Lou Hom >K'93			     
lhom at 	    

More information about the Methods mailing list