pH adjustment in Tris-HCl buffer?
lhom at OCF.Berkeley.EDU
Fri Feb 24 08:50:25 EST 2006
I'd say the preferred method is to start with Tris base and then adjust
downward with HCl. Alternatively, you could take a solution of Tris-HCl
and a solution of Tris base of the same concentration and mix them until
you reach the desired pH. Adding NaOH (essentially backtitrating an
overshot) leads to a higher concentration of NaCl than you intend, which
may or may not have actual effects on your experiment.
Lou Hom >K'93
lhom at ocf.berkeley.edu
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