pH adjustment in Tris-HCl buffer?

[Louis Hom]

I'd say the preferred method is to start with Tris base and then adjust 
downward with HCl.  Alternatively, you could take a solution of Tris-HCl 
and a solution of Tris base of the same concentration and mix them until 
you reach the desired pH.  Adding NaOH (essentially backtitrating an 
overshot) leads to a higher concentration of NaCl than you intend, which 
may or may not have actual effects on your experiment.
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______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/