Stability of sodium carbonate buffer

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Fri Feb 24 09:15:08 EST 2006


Han wrote:

  After a few hours of opening 
> and closing the tube with PRP, some/much of the dissolved CO2 is lost, the 
> buffering of the PRP goes awry (I think losing CO2 will acidify), and 
> platelet reactivity is greatly diminished.  To prevent/delay this, we blow 
> 5% CO2 in air over the plasma.

CO2 stands in equilibrium with H2CO3, carbonic acid. Removing an acid
however increases pH (making the solution more alkaline).


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