Dh5 alpha cultures not viable ?
blackhole at abuse.plus.com
Fri Feb 24 12:15:52 EST 2006
Historians believe that in newspost
<mailman.1224.1140796133.29584.methods at net.bio.net> on Fri, 24 Feb 2006,
Siddhartha Parida <sidharth4 at gmail.com> penned the following literary
>I had ordered a culture of DH5alpha from a supplier and when i
>inoculated it in a LA + Amp (10mg/ml) the culture was able to grow,
DH5alpha should not be able to grow on its own without an Amp resistant
plasmid, on Amp plates. It is Amp sensitive.
>streaked it into slants and keep it at 4C for a month but now when i
>want to work on it again, the culture is not growing , i have repeated
>ot many times but without sucess.
I store all strains stocks at -70C in 2 volumes LB plus 1 volume 50%
(v/v) glycerol. My >25year old stocks are fine. -20C as glycerol stocks
or 7% DMSO stocks IMHO is better than storage at 4C. I'm sure I still
have some 10year old room temp stocks as stabs in 0.7% agar LB in screw
caps eppendorfs which were wax oversealed and they are still viable. For
real safety, lyophilise.
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
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