Dh5 alpha cultures not viable ?

Jayakumar, R R.Jayakumar at roswellpark.org
Fri Feb 24 12:45:58 EST 2006

Well.. if E.coli had the ability to sporulate (like Bacillus), then it would survived a month at 4C.  Unfortunately it does not.  But I have seen miracles happen.  
  bye and best of luck

-----Original Message-----
From: methods-bounces at oat.bio.indiana.edu
[mailto:methods-bounces at oat.bio.indiana.edu]On Behalf Of Siddhartha
Sent: Friday, February 24, 2006 12:20 AM
To: Methods at magpie.bio.indiana.edu
Subject: Dh5 alpha cultures not viable ?

I had ordered a culture of DH5alpha from a supplier and when i
inoculated it in a LA + Amp (10mg/ml) the culture was able to grow, i
streaked it into slants and keep it at 4C for a month but now when i
want to work on it again, the culture is not growing , i have repeated
ot many times but without sucess.

Point to be noted: That our water bath shaker is out of order so i had
kept the flask containing the media in a bacterialogical incubator at
37C, is vigorous shakes a must for revival of these culture ?

Sidharth Parida
Senior Faculty
Center for biotechnology and Research
Neelachal Inst of Medical Sciences
O.C.H.C building , Near Ram Mandir
sidharth4 at gmail.com

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