Dh5 alpha cultures not viable ?
freisinger at aci.unizh.ch
Fri Feb 24 13:31:22 EST 2006
DH5alpha should not grow on amp in the first place because it does not
carry resistancy against it... If you mean you had DH5alpha cells with a
vector in it carrying amp resistancy, maybe this vector expresses a toxic
protein? Some of my bacteria do not survive longer than 1 or 2 month on a
plate with a vector having certain inserts. It's always a good idea to keep
glycerol stocks. Even better to have the plasmid isolated.
At 06:19 24.02.2006, Siddhartha Parida wrote:
>I had ordered a culture of DH5alpha from a supplier and when i
>inoculated it in a LA + Amp (10mg/ml) the culture was able to grow, i
>streaked it into slants and keep it at 4C for a month but now when i
>want to work on it again, the culture is not growing , i have repeated
>ot many times but without sucess.
>Point to be noted: That our water bath shaker is out of order so i had
>kept the flask containing the media in a bacterialogical incubator at
>37C, is vigorous shakes a must for revival of these culture ?
>Center for biotechnology and Research
>Neelachal Inst of Medical Sciences
>O.C.H.C building , Near Ram Mandir
>sidharth4 at gmail.com
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