DNA extraction from olive oil

Deanne Bell dbell at fresno.ars.usda.gov
Fri Feb 24 15:02:38 EST 2006


Hi

The best method of genomic DNA extraction that I have found for oily
stuff is a protocol from J. Prince getting DNA from peppers (Capsicum).
Sorry, can't remember the citation.

What kind of DNA are you looking for - just curious?

Deanne Bell

 
 
 

>-----Original Message-----
>From: methods-bounces at oat.bio.indiana.edu 
>[mailto:methods-bounces at oat.bio.indiana.edu] On Behalf Of 
>Giulio Paradisi
>Sent: Friday, February 24, 2006 9:57 AM
>To: methods at magpie.bio.indiana.edu
>Subject: DNA extraction from olive oil
>
>Hi, 
>
> 
>
>I need help for DNA extraction from olive oil. I would use 
>this for DNA fingerprinting by using microsatellite markers . 
>I tried some methods as CTAB (Doyle&Doyle 1990) and some 
>commercial KITS as Macherey Naghel Spin food  but I have not 
>obtained good yealds. Thanks
>
> 
>
> ----------------------------------------------------
>
>> *Giulio Paradisi***
>
>> 
>
>> Department of Environmental Sciences "G.Sarfatti"
>
>> Section of Plant Biology - U.R. Plant Biotechnology University of
>
>> Siena Via P.A. Mattioli,4 53100 Siena - ITALY Tel. 039-0577-232841
>
>> 
>
>> Gsm:+393281125398
>
>> Fax. 039-0577-232860
>
>> email: gparadisi at unisi.it
>
>> Department web site:
>
>>  <http://www.unisi.it/ricerca/dip/dba/index_eng.htm>
>http://www.unisi.it/ricerca/dip/dba/index_eng.htm
>
>> 
>
>> ---------------------------------------------------------
>
> 
>
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