Stability of sodium carbonate buffer
Han
nobody at nospam.not
Sat Feb 25 07:57:09 EST 2006
Dr Engelbert Buxbaum <engelbert_buxbaum at hotmail.com> wrote in
news:dtn4df$ogi$01$1 at news.t-online.com:
> Han wrote:
>
> After a few hours of opening
>> and closing the tube with PRP, some/much of the dissolved CO2 is
>> lost, the buffering of the PRP goes awry (I think losing CO2 will
>> acidify), and platelet reactivity is greatly diminished. To
>> prevent/delay this, we blow 5% CO2 in air over the plasma.
>
> CO2 stands in equilibrium with H2CO3, carbonic acid. Removing an acid
> however increases pH (making the solution more alkaline).
>
That's what I thought too, but I need to check the real world some time.
Platelet aggregation goes out the window upon acidification. But I have to
check.
--
Best regards
Han
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