Stability of sodium carbonate buffer

Han nobody at nospam.not
Sat Feb 25 07:57:09 EST 2006


Dr Engelbert Buxbaum <engelbert_buxbaum at hotmail.com> wrote in
news:dtn4df$ogi$01$1 at news.t-online.com: 

> Han wrote:
> 
>   After a few hours of opening 
>> and closing the tube with PRP, some/much of the dissolved CO2 is
>> lost, the buffering of the PRP goes awry (I think losing CO2 will
>> acidify), and platelet reactivity is greatly diminished.  To
>> prevent/delay this, we blow 5% CO2 in air over the plasma.
> 
> CO2 stands in equilibrium with H2CO3, carbonic acid. Removing an acid
> however increases pH (making the solution more alkaline).
> 
That's what I thought too, but I need to check the real world some time.  
Platelet aggregation goes out the window upon acidification.  But I have to 
check.


-- 
Best regards
Han 
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