oligo hybridization conditions for northern

Josmar josmarlangner at yahoo.de
Sun Feb 26 12:39:54 EST 2006


Dear all,
does anyone have the buffer conditions handy to hybridize hot oligo
probes (~20-mers) on RNA blotted and UV-crosslinked to nylon membranes.
I would assume conditions should be pretty native, as they would be in
a PCR. Unincorporated hot gamma-ATP is removed by gelfiltration. What
would be best way to block the membrane against unspecific binding of
the probe? Salmon sperm DNA?
Thanks
Josmar



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