what is the difference between DH5alpha and TOP10 E.coli cells?

as nospam at nospamss.dk
Sun Feb 26 14:20:35 EST 2006


"ali" <atahery at gmail.com> skrev i en meddelelse 
news:1140796893.681532.48280 at i39g2000cwa.googlegroups.com...
> Looking for the difference between these two strains. Anybody has an
> idea?

Genotype (TOP10): F- mcrA D(mrr-hsdRMS-mcrBC) f80lacZDM15 DlacX74 deoR recA1 
araD139 D(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG

E. coli DH5?:
F-, ?80dlacZ?M15, ?(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk-, mk+), 
phoA, supE44, ?-, thi-1, gyrA96, relA1

In my hands they behave quite different.

A.S.
Denmark (Sorry) 




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