Primers, DDWater, TE Buffer

Christian Praetorius prae at gmx.net
Mon Feb 27 16:37:16 EST 2006


"Alvin Alejandrino" <aalejandrino at nhm.org> wrote:

>Normally, I use TE Buffer to dilute and resuspend primers from the manufacturer.  Some people have told me that Double Distilled Water is an okay and easy way to dilute the primers.  Any thoughts?  It seems way too easy.  What problems will I encounter?

I think, it doesn't matter. TE may be better because it is buffered
and my complex ions, which are necessary for nucleases. I always
dissolve and dilute my primers in double distilled water and this
worked always.

Christian

-- 
        [X] <-- nail here for new monitor


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