Primers, DDWater, TE Buffer

Rajesh Kumar Singh rsingh at EMBL-Hamburg.DE
Tue Feb 28 04:05:12 EST 2006

usually its not a problem in distilled water. but its good to
keep track of pH of water being used.

Rajesh Kumar Singh
European Molecular Biology Laboratory
C/o DESY, Notkestrasse 85, Geb. 25A
22603 Hamburg, Germany
E-mail: rsingh at

On Mon, 27 Feb 2006, Christian Praetorius wrote:

> "Alvin Alejandrino" <aalejandrino at> wrote:
> >Normally, I use TE Buffer to dilute and resuspend primers from the manufacturer.  Some people have told me that Double Distilled Water is an okay and easy way to dilute the primers.  Any thoughts?  It seems way too easy.  What problems will I encounter?
> I think, it doesn't matter. TE may be better because it is buffered
> and my complex ions, which are necessary for nucleases. I always
> dissolve and dilute my primers in double distilled water and this
> worked always.
> Christian
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