Primers, DDWater, TE Buffer

GysdeJongh jongh711 at planet.nl
Tue Feb 28 10:06:08 EST 2006


"Christian Praetorius" <prae at gmx.net> wrote in message 
news:46j1piFbdg6eU1 at individual.net...
> Christian Praetorius <prae at gmx.net> wrote:
>
>>I always
>>dissolve and dilute my primers in double distilled water and this
>>worked always.
>
> One more thing: I always dilute the primer stocks to 100pmol and make
> a 10pmol dilution for use in PCR.

Hi,
I  _think_  you mean 100 pmol / microliter , which is 100 micromolar or 100 
micromol / liter
To avoid the greek alphabet  :)
hth
Gys




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