agarose gel purification of cloning vector

Tom Knight tk at csail.mit.edu
Tue Jan 3 15:51:28 EST 2006


mwcrepeau at hawaii.rr.com writes:

> In cutting out the band I work as quickly as possible, but it still
> probably takes 30 seconds.  I've never timed it.  I imagine
> overexposure might reduce cloning efficiency, but even with extreme
> overexposure isn't no colonies rather surprising?

The damage by UV is highly dependent on the wavelength.  254 nm light
is essentially instantly destructive.  305 nm light is questionable,
while 365 nm light is tolerable for some length of time.  You might
want to look into the Clare "Dark Reader."



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