Question about streptomycin sulfate precipitation
blackhole at abuse.plus.com
Thu Jan 5 06:01:33 EST 2006
Historians believe that in newspost
<mailman.650.1136330661.29584.methods at net.bio.net> on Tue, 3 Jan 2006,
Igal Finarov <igal.finarov at weizmann.ac.il> penned the following literary
>I saw in Internet that you had the same question I have: what is the
>percent of streptomycin sulfate needed to precipitate DNA. Can you
>please share with me your knowledge on that issue.
Find any 1960's or early 70's molecular biology enzyme purification
paper and you will invariably find that they used Streptomycin sulphate
pptation prior to loading their first column. Subsequently Polymin P
pptation came along. Just be very careful about pptating the protein you
may want with too much of either. You can't always back recover it.
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
More information about the Methods