Gateway cloning and expression

Simon Dawson simon.dawson at nottingham.ac.uk
Thu Jan 12 09:12:35 EST 2006


Hi,
	Does anyone out there have experience with the Gateway cloning system 
of Invitrogen? Anyone tried using there vectors pcDNA-DEST40 and 
pcDNA-DEST47?
	I've tried any number of cDNAs in them and get no expression on 
transfection into mammalian cells. Sequenced them and they're all OK. 
I'd be interested to hear what experiences anyone else out there has had 
with the GateWay system.
	Cheers,


		Simon.


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