Gateway cloning and expression
simon.dawson at nottingham.ac.uk
Thu Jan 12 09:12:35 EST 2006
Does anyone out there have experience with the Gateway cloning system
of Invitrogen? Anyone tried using there vectors pcDNA-DEST40 and
I've tried any number of cDNAs in them and get no expression on
transfection into mammalian cells. Sequenced them and they're all OK.
I'd be interested to hear what experiences anyone else out there has had
with the GateWay system.
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