PCR optimization

[Duncan Clark]

Historians believe that in newspost 
<1137469560.315751.95860 at g49g2000cwa.googlegroups.com> on Mon, 16 Jan 
2006, vagos <el36 at le.ac.uk> penned the following literary masterpiece:
>Have you got any ideas on how I could improve on this?

I would try using a left hand primer that ends with the A mismatch at 
it's 3' end. Preferably use that with a proofreading polymerase i.e. 
Pfu or a.n.other.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.