Enzyme to digest dsDNA in ss/ds mix

Susan Hogarth hogarth at gmail.com
Thu Jan 19 11:05:06 EST 2006


Nick Theodorakis wrote:
> On 18 Jan 2006 15:37:40 -0800, "Susan Hogarth" <hogarth at gmail.com>
> wrote:
>
> >Is there an enzyme which will digest dsDNA in a ss/ds mix?
> >
>
> Exonuclease III might be suitable for your needs. What is your
> substrate(s) do yo have and what products do you need?

I have the products of an asymmetric PCR, and want only the ss product
(or at least to enrich it in proportion to the ds product.

- Susan



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