Enzyme to digest dsDNA in ss/ds mix

Dag Rune Gjellesvik dagrg_nospam at online.no
Tue Jan 24 06:40:34 EST 2006


"Susan Hogarth" <hogarth at gmail.com> skrev i melding
news:1137627460.149656.27720 at g14g2000cwa.googlegroups.com...
> Is there an enzyme which will digest dsDNA in a ss/ds mix?
>
> Thanks!
>
> - Susan
>
Hi, Susan,
There is one enzyme that does just that. Shrimp nuclease is very active on
dsDNA, while ssDNA is essentially left intact (5,000-fold specificity).

You may get it from either USB (in US) or GE in the rest of the world.

Best Regards,
Dag Rune Gjellesvik





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