phosphocellulose slurries for protein purification on gravity column

Mike Autry jma at ddt.biochem.umn.edu
Wed Jan 25 13:50:22 EST 2006


Hi Methods,

Are pre-hydrated phosphocellulose slurries commercially available?  

What is the difference between mesh and fibrous forms of
phosphocellulose resin?

Any recommendations on company/brand type?

This website for tubulin purification has a protocol for rehydrating dry
resin and recommends pre-blocking column with BSA (to block irreversible
binding sites).

http://mitchison.med.harvard.edu/protocols/tubprep.html#III.%20%20Pourin
g%20a%201L%20Phosphocellulose%20(PC)%20Column

I will be purifying a 110 kDa membrane protein solubilized in non-ionic
detergent.

Thanks,

Mike Autry
Research Associate with Dave Thomas
http://ddt.biochem.umn.edu/


More information about the Methods mailing list