phosphocellulose slurries for protein purification on gravity column

Mike Autry jma at
Wed Jan 25 13:50:22 EST 2006

Hi Methods,

Are pre-hydrated phosphocellulose slurries commercially available?  

What is the difference between mesh and fibrous forms of
phosphocellulose resin?

Any recommendations on company/brand type?

This website for tubulin purification has a protocol for rehydrating dry
resin and recommends pre-blocking column with BSA (to block irreversible
binding sites).

I will be purifying a 110 kDa membrane protein solubilized in non-ionic


Mike Autry
Research Associate with Dave Thomas

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