PCR problem, need your help

BioMale amirnach at post.tau.ac.il
Wed Jan 25 18:31:40 EST 2006


Hi everyone,
i need to amplify the sequence of nitric oxide synthase(from chick),
but it seems to be impossible due to the fact that the 3' and 5' are
really poor regions to locate a primer, and any primer for those
regions is interfer with many other sequences (as BLAST reveal).
the sequence is predicted, and i don't know if the mRNA starting at the
first base and/or ends at the last one.
i'll love to hear any suggestion or comment,
Amir



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