Hind III Digest of PCR Products
aawara at nohome.org
Sun Jan 29 14:54:03 EST 2006
In <mailman.891.1138561310.29584.methods at net.bio.net>,
Mark Blenner <markblenner at yahoo.com> wrote:
> I have a 790 bp PCR fragment I am trying to ligate
> into a 7400bp vector - some data suggests there may be
> some difficulties in cutting the HindIII at the end
> of my PCR fragment, but I've added 6 extra basepairs
> and still doesnt work. Anyone seen this before?
Usually not - what's the base composition of the terminal 6 bp? In any event,
here's one solution. Phosphoryate the oligos before PCR. Purify the PCR
product, and self-ligate it. Now cut this with HindIII.
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but nothing to choose from - Peter Ustinov.
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