alpha complementation problem ?

Siddhartha Parida sidharth4 at
Mon Jan 30 07:14:40 EST 2006

I have been tryin to plate Dh5 alpha on to a LBA plate with X-gal and
IPTG but the blue colonies are not coming ?

Is the bug containg the plasmid not stable ? or the x-gal  and IPTG
not woking cause they are 1 yr old solutions ?

Sidharth Parida
Senior Faculty
Center for biotechnology and Research
Neelachal Inst of Medical Sciences
O.C.H.C building , Near Ram Mandir
sidharth4 at

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