KUMAR MANOJ (MR.)
KumarManoj at iocrd.co.in
Sat Jul 1 00:39:47 EST 2006
>From internet I could know that you are using CSGE in which is based on a
similar principle as DGGE but can apply on a conventional manual sequencing
I aimed to ampify the total soil DNA and follwed by Denaturing gradient gel
electrophoresis (DGGE) to determine the diversity of microbial communities.
* Can the CSGE be used for this purpose.
* I am having Hoefer SQ3 manual sequencing gel apparatus. Can I use
that system for the said purpose and what would the changes required and
Looking forward to have your valuable guidence
Thanking you in advace
Indian Oil (R&D)
Sector-13 Faridabad-121007 INDIA
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